Rabbit anti-PLEKHG2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-414A maps to a region between residue 550 and 600 of human pleckstrin homology domain containing, family G (with RhoGef domain) member 2 using the numbering given in entry NP_073746.2 (GeneID 64857).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PLEKHG2 immobilized on solid support.
The epitope recognized by A302-414A-T maps to a region between residue 550 and 600 of human pleckstrin homology domain containing, family G (with RhoGef domain) member 2 using the numbering given in entry NP_073746.2 (GeneID 64857).
Additional Product Information
PLEKHG2 (pleckstrin homology domain-containing family G member 2) contains a RhoGEF/DH domain and a pleckstrin homology (PH) domain. PH domains are found in proteins involved in cell signaling. PLEKHG2 is proposed to be a guanine-nucleotide exchange factor (GEF) for RAC1/CDC42.
Alternate Names
ARHGEF42; CLG; common-site lymphoma/leukemia guanine nucleotide exchange factor; CTB-60E11.4; LDAMD; PH domain-containing family G member 2; pleckstrin homology domain containing, family G (with RhoGef domain) member 2; pleckstrin homology domain-containing family G member 2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.