Rabbit anti-PolD2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-322A maps to a region between residue 419 to 469 of human Polymerase (DNA directed), Delta 2, Accessory Subunit using the numbering given in entry NP_001120690.1 (GeneID 5425).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PolD2 immobilized on solid support.
The epitope recognized by A304-322A-T maps to a region between residue 419 to 469 of human Polymerase (DNA directed), Delta 2, Accessory Subunit using the numbering given in entry NP_001120690.1 (GeneID 5425).
Additional Product Information
DNA polymerase delta subunit 2 (PolD2) is the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. PolD2 is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA [taken from NCBI Entrez Gene (Gene ID: 5425)].
Alternate Names
DAN polymerase delta 2, accessory subunit; DNA polymerase delta subunit 2; DNA polymerase delta subunit p50; Pol delta B subunit (p50); polymerase (DNA directed), delta 2, accessory subunit; polymerase (DNA directed), delta 2, regulatory subunit 50kDa; polymerase (DNA) delta 2, accessory subunit
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.