Rabbit anti-POLR1A/RPA194 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
POLR1A is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is the largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O95602].
Alternate Names
A190; AFDCIN; DNA-directed RNA polymerase I largest subunit; DNA-directed RNA polymerase I subunit A; DNA-directed RNA polymerase I subunit A1; DNA-directed RNA polymerase I subunit RPA1; polymerase (RNA) I polypeptide A, 194kDa; polymerase (RNA) I subunit A; RNA polymerase I 194 kDa subunit; RNA polymerase I subunit A1; RPA1; RPA194; RPO14; RPO1-4
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.