Rabbit anti-PPM1G Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-881A maps to a region between residue 500 and the C-terminus (residue 546) of human Protein Phosphatase Magnesium-dependent 1 Gamma using the numbering given in entry NP_817092.1 (GeneID 5496).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PPM1G immobilized on solid support.
The epitope recognized by A300-881A-T maps to a region between residue 500 and the C-terminus (residue 546) of human Protein Phosphatase Magnesium-dependent 1 Gamma using the numbering given in entry NP_817092.1 (GeneID 5496).
Additional Product Information
Members of the protein phosphatase 2C (PP2C) family of isoenzymes are serine/threonine protein phosphatases that act as monomers, are magnesium or manganese dependent, and are structurally distinct from PP1, PP2A, and PP2B protein phosphatases. There are 14 mammalian PP2C genes that encode for members of the PP2C family of isoenzymes. Members of this family include PP2C alpha (PPM1A), PP2C beta (PPM1B), PP2C gamma (PPM1G), and PP2C delta (PPM1D). Because PPM1G has been shown to bind and dephosphorylate histones H2A and H2B, it appears to play a role in chromatin remodeling in response to DNA damage.
Alternate Names
PP2CG; PP2CGAMMA; PP2C-gamma; PPP2CG; protein phosphatase 1C; protein phosphatase 1G; protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform; protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase 2C gamma isoform; protein phosphatase 2C isoform gamma; protein phosphatase magnesium-dependent 1 gamma
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.