Rabbit anti-PPP1CC IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00485 maps to a region between residue 280 and the C-terminus (residue 323) of human Protein Phosphatase 1, Catalytic Subunit, Gamma Isoform using the numbering given in entry NP_002701.1 (GeneID 5501).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PPP1CC immobilized on solid support.
The epitope recognized by IHC-00485-T maps to a region between residue 280 and the C-terminus (residue 323) of human Protein Phosphatase 1, Catalytic Subunit, Gamma Isoform using the numbering given in entry NP_002701.1 (GeneID 5501).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Protein phosphatase 1 (PP1) is a major eukaryotic serine/threonine phosphatase that is involved in a multitude of cellular functions. PP1 is a holoenzyme that consists of the PP1 catalytic subunit (PP1c) that associates with and is regulated by over 50 regulatory subunits. There are several gene and alternative splice products of the catalytic subunit PP1c. PPP1CC encodes the PP1 gamma catalytic subunit. In addition to PP1 gamma, there are two splice variants of PP1 alpha (PPP1CA), and a PP1 beta (PPP1CB) form. The mutually exclusive interaction between the various catalytic and regulatory subunits allows the PP1 enzyme to be involved in a variety of functions which include cell division, glycogen metabolism, muscle contractility, and protein synthesis. serine/threonine-protein phosphatase PP1-gamma catalytic subunit, PP-1G, PPP1G.
Alternate Names
PP1C; PP-1G; PPP1G; protein phosphatase 1, catalytic subunit, gamma isoform; protein phosphatase 1, catalytic subunit, gamma isozyme; protein phosphatase 1C catalytic subunit; serine/threonine phosphatase 1 gamma; serine/threonine-protein phosphatase PP1-gamma catalytic subunit
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.