Rabbit anti-PPP2R5C Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-814A maps to a region between residue 490 and 524 of human Protein Phosphatase 2, Regulatory Subunit B', Gamma using the numbering given in entry NP_002710.2 (GeneID 5527).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PPP2R5C immobilized on solid support.
The epitope recognized by A303-814A-T maps to a region between residue 490 and 524 of human Protein Phosphatase 2, Regulatory Subunit B', Gamma using the numbering given in entry NP_002710.2 (GeneID 5527).
Additional Product Information
Protein phosphatase 2, regulatory subunit B', gamma (PPP2R5C) is a regulatory subunit of the PP2A phosphatase. The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may specifically dephosphorylate and activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13362].
Alternate Names
B' alpha regulatory subunit; B56G; B56gamma; PP2A B subunit isoform B56-gamma; PP2A B subunit isoform B'-gamma; PP2A B subunit isoform PR61-gamma; PP2A B subunit isoform R5-gamma; PR61G; protein phosphatase 2 regulatory subunit B', gamma; protein phosphatase 2, regulatory subunit B (B56), gamma isoform; protein phosphatase 2, regulatory subunit B', gamma isoform; renal carcinoma antigen NY-REN-29; serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.