Rabbit anti-PPP3CA Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-907A maps to a region between residue 1 and 50 of human Protein Phosphatase 3, Catalytic Subunit, Alpha isoform using the numbering given in entry NP_000935.1 (GeneID 5530).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PPP3CA immobilized on solid support.
The epitope recognized by A300-907A-T maps to a region between residue 1 and 50 of human Protein Phosphatase 3, Catalytic Subunit, Alpha isoform using the numbering given in entry NP_000935.1 (GeneID 5530).
Additional Product Information
PPP3CA encodes the protein phosphatase 2B (PP2B) alpha catalytic subunit. PP2B, also known as calcineurin, is a holoenzyme that is comprised of a catalytic subunit associated with regulatory subunits. PP2B is a calcium regulated enzyme that is activated by calmodulin and participates in the signaling cascades involved in development of the nervous, cardiovascular, and musculoskeletal systems.
Alternate Names
calcineurin A alpha; calmodulin-dependent calcineurin A subunit alpha isoform; CALN; CALNA; CALNA1; CAM-PRP catalytic subunit; CCN1; CNA1; IECEE; IECEE1; PPP2B; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform; protein phosphatase 3 catalytic subunit alpha isozyme; serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.