Rabbit anti-PRCC IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00675 maps to a region between residue 125 and 175 of human papillary renal cell carcinoma (translocation-associated) using the numbering given in entry NP_005964.3 (GeneID 5546).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PRCC immobilized on solid support.
The epitope recognized by IHC-00675-T maps to a region between residue 125 and 175 of human papillary renal cell carcinoma (translocation-associated) using the numbering given in entry NP_005964.3 (GeneID 5546).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
In a subset of papillary renal cell carcinomas, a t(X;1)(p11;q21) chromosome translocation has been repeatedly reported and is thought to be the cause of the cancer. As a result of the translocation, the transcription factor TFE3 on the X chromosome becomes fused to the PRCC (papillary renal cell carcinoma translocation-associated) gene on chromosome 1. The fused gene results in the fusion of N-terminal proline-rich region of the protein encoded by this gene to the entire TFE3 protein. This protein has been shown to interact with the mitotic checkpoint protein MAD2B, which suggests that the dominant-negative effect of the fusion protein with TFE3 may lead to a mitotic checkpoint defect [taken from NCBI GeneID:5546].
Alternate Names
papillary renal cell carcinoma (translocation-associated); papillary renal cell carcinoma translocation-associated gene protein; PRCC, proline rich mitotic checkpoint control factor; proline-rich protein PRCC; RCCP1; TPRC
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.