Rabbit anti-PRIM2/DNA Primase Large Subunit Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-274A maps to a region between residue 459 to 509 of human DNA primase large subunit using the numbering given in entry P49643.2 (GeneID 5558).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PRIM2/DNA Primase Large Subunit immobilized on solid support.
The epitope recognized by A305-274A-T maps to a region between residue 459 to 509 of human DNA primase large subunit using the numbering given in entry P49643.2 (GeneID 5558).
Additional Product Information
PRIM2 is the 58 kilodalton large subunit of DNA primase. DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P49643].
Alternate Names
DNA primase 58 kDa subunit; DNA primase large subunit; p58; PRIM2A; primase (DNA) subunit 2; primase, DNA, polypeptide 2 (58kDa)
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.