Rabbit anti-PRKCQ Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-233A maps to a region between residue 656 to 706 of human Protein Kinase C, Theta using the numbering given in entry NP_006248.1 (GeneID 5588).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PRKCQ immobilized on solid support.
The epitope recognized by A304-233A-T maps to a region between residue 656 to 706 of human Protein Kinase C, Theta using the numbering given in entry NP_006248.1 (GeneID 5588).
Additional Product Information
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. Protein kinase C theta type (PRKCQ) is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factor [taken from NCBI Entrez Gene (Gene ID: 5588)].
Alternate Names
nPKC-theta; PRKCT; protein kinase C theta type
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.