Rabbit anti-PRPF8 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-922A maps to a region between residue 2285 and 2335 of human Pre-mRNA-Processing Factor 8 using the numbering given in entry NP_006436.3 (GeneID 10594).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PRPF8 immobilized on solid support.
The epitope recognized by A303-922A-T maps to a region between residue 2285 and 2335 of human Pre-mRNA-Processing Factor 8 using the numbering given in entry NP_006436.3 (GeneID 10594).
Additional Product Information
Pre-mRNA-processing-splicing factor 8 (PRPF8) is a central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. PRPF8 interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8TCS8].
Alternate Names
220 kDa U5 snRNP-specific protein; apoptosis-regulated protein 1; apoptosis-regulated protein 2; HPRP8; p220; precursor mRNA processing protein; pre-mRNA-processing-splicing factor 8; PRP8; PRP8 homolog; PRP8 pre-mRNA processing factor 8 homolog; PRPC8; RP13; SNRNP220; splicing factor Prp8; U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.