Rabbit anti-PSMB8/LMP7 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-195A-T maps to a region between residue 226 to 276 of human Proteasome subunit beta type-8 using the numbering given in entry P28062.3 (GeneID 5696).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PSMB8 immobilized on solid support.
The epitope recognized by A305-195A maps to a region between residue 226 to 276 of human Proteasome subunit beta type-8 using the numbering given in entry P28062.3 (GeneID 5696).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Proteasomes are distributed throughout eukaryotic cells and cleave peptides in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The gene PSMB8 (aka LMP7) encodes a member of the proteasome B-type family, proteasome subunit beta type-8, that is a 20S core beta subunit. Expression of PSMB8/LMP7 is induced by gamma interferon and the PSMB8 gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Nakajo syndrome, an autosomal recessive autoinflammatory disorder, is caused by mutations to PSMB8 [taken from NCBI Entrez Gene (Gene ID: 5696) and UniProtKB (Entry: P28062)].
Alternate Names
ALDD; D6S216; D6S216E; JMP; LMP7; low molecular mass protein 7; low molecular weight protein 7; macropain subunit C13; multicatalytic endopeptidase complex subunit C13; NKJO; PRAAS1; protease component C13; proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7); proteasome catalytic subunit 3i; proteasome component C13; proteasome subunit beta 5i; proteasome subunit beta type-8; Proteasome subunit beta-5i; proteasome subunit Y2; proteasome-related gene 7; PSMB5i; really interesting new gene 10 protein; RING10
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.