Rabbit anti-PSME3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-879A-T maps to a region between residue 204 and 254 of human Proteasome (Prosome, Macropain) Activator Subunit 3 using the numbering given in entry NP_005780.2 (GeneID 10197).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PSME3 immobilized on solid support.
The epitope recognized by A303-879A maps to a region between residue 204 and 254 of human Proteasome (Prosome, Macropain) Activator Subunit 3 using the numbering given in entry NP_005780.2 (GeneID 10197).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME3 [proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)] is the gamma subunit of the 11S regulator [taken from NCBI Entrez Gene (Gene ID: 10197)].
Alternate Names
11S regulator complex gamma subunit; 11S regulator complex subunit gamma; activator of multicatalytic protease subunit 3; epididymis secretory protein Li 283; HEL-S-283; Ki; Ki antigen; Ki nuclear autoantigen; PA28 gamma variant 5; PA28G; PA28gamma; PA28-gamma; proteasome (prosome, macropain) activator subunit 3 (PA28 gamma); proteasome activator 28 subunit gamma; proteasome activator 28-gamma; proteasome activator complex subunit 3; REG gamma-3; REG-GAMMA
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.