Rabbit anti-Pumilio 1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-576A maps to a region between residue 1 and 50 of human Pumilio 1 using the numbering given in entry NP_055491.1 (GeneID 9698).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Pumilio 1 immobilized on solid support.
The epitope recognized by A302-576A-T maps to a region between residue 1 and 50 of human Pumilio 1 using the numbering given in entry NP_055491.1 (GeneID 9698).
Additional Product Information
Pumilio 1 is an evolutionarily conserved RNA binding protein that functions as a translational regulator of genes involved in embryogenesis and germline cell development. Pumilio 1, which is expressed in many tissue types, is homologous to the germline specific pumilio 2.
Alternate Names
HSPUM; PUMH; PUMH1; pumilio homolog 1; pumilio-1; PUML1; SCA47
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.