Rabbit anti-Pumilio 2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-202A maps to a region between residues 100 and 150 of human Pumilio 2 using the numbering given in entry NP_056132.1 (GeneID 23369).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Pumilio 2 immobilized on solid support.
The epitope recognized by A300-202A-T maps to a region between residues 100 and 150 of human Pumilio 2 using the numbering given in entry NP_056132.1 (GeneID 23369).
Additional Product Information
Pumilio 2 is a germline specific RNA binding protein that functions as a translational regulator by binding 3’ UTR elements. Pumilio 2 and its homolog Pumiolio 1 are highly evolutionarily conserved proteins that appear to function in germline maintenance. Pumilio 2 has been shown to interact with DAZL, a protein required for germ cell development.
Alternate Names
PUMH2; pumilio homolog 2; pumilio-2; PUML2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.