Rabbit anti-Pygo2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-730A maps to a region between residue 1 and 50 of human Pygopus 2 using the numbering given in entry NP_612157.1 (GeneID 90780).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Pygo2 immobilized on solid support.
The epitope recognized by A302-730A-T maps to a region between residue 1 and 50 of human Pygopus 2 using the numbering given in entry NP_612157.1 (GeneID 90780).
Additional Product Information
Pygo 2 is a member of the Pygopus family of genes first identified in Drosophila. Two pygo genes exist in mammals: Pygo1 and Pygo2. Pygo genes have been found to be involved in the regulation of Wnt signaling.
Alternate Names
1190004M21Rik; pygopus 2; pygopus homolog 2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.