Rabbit anti-QKI Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-183A maps to the C-terminus of isoform 5 of human quaking homolog according to entry AAH19917.1 (GeneID 9444). The epitope is not found in isoforms 1, 2, 3, 4, 6, 7 or 7B according to TrEMBL entries Q9P0Y1, Q9P0Y0, Q9P0X6, Q9P0X8, Q96PU7, Q969L9 and Q96PU6, respectively.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to QKI immobilized on solid support.
The epitope recognized by A300-183A-T maps to the C-terminus of isoform 5 of human quaking homolog according to entry AAH19917.1 (GeneID 9444). The epitope is not found in isoforms 1, 2, 3, 4, 6, 7 or 7B according to TrEMBL entries Q9P0Y1, Q9P0Y0, Q9P0X6, Q9P0X8, Q96PU7, Q969L9 and Q96PU6, respectively.
Additional Product Information
QKI is an RNA-binding protein and translational repressor important to embryogenesis and myelination. QKI exist as multiple isoforms with distinct functions that regulate pre-mRNA splicing and the stability of proteins central to oligodendrocyte differentiation. QKI has been identified as a candidate gene in schizophrenia.
Alternate Names
homolog of mouse quaking QKI (KH domain RNA binding protein); Hqk; hqkI; protein quaking; QK; QK1; QK3; QKI/LOC100132735 fusion; quaking homolog, KH domain RNA binding; RNA binding protein HQK
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.