Rabbit anti-RACK1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-545A maps to a region between residue 1 and 50 of human receptor of activated protein kinase C 1 using the numbering given in entry NP_006089.1 (GeneID 10399).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RACK1 immobilized on solid support.
The epitope recognized by A302-545A-T maps to a region between residue 1 and 50 of human receptor of activated protein kinase C 1 using the numbering given in entry NP_006089.1 (GeneID 10399).
Additional Product Information
RACK1 (receptor of activated protein kinase C1) was originally identified as a factor that could bind the activated form of protein kinase C. It contains seven WD40 motifs that interact with various signaling molecules.
Alternate Names
cell proliferation-inducing gene 21 protein; GNB2L1; Gnb2-rs1; guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1; guanine nucleotide binding protein beta polypeptide 2-like 1; guanine nucleotide-binding protein subunit beta-2-like 1; guanine nucleotide-binding protein subunit beta-like protein 12.3; H12.3; HLC-7; human lung cancer oncogene 7 protein; lung cancer oncogene 7; PIG21; proliferation-inducing gene 21; protein homologous to chicken B complex protein, guanine nucleotide binding; Receptor for activated C kinase; receptor of activated protein C kinase 1; receptor of activated protein kinase C 1; small ribosomal subunit protein RACK1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.