Rabbit anti-RALY Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-069A-T maps to a region between residue 150 and 200 of human hnRNP-associated with lethal yellow homolog using the numbering given in entry NP_057951.1 (GeneID 22913).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RALY immobilized on solid support.
The epitope recognized by A302-069A maps to a region between residue 150 and 200 of human hnRNP-associated with lethal yellow homolog using the numbering given in entry NP_057951.1 (GeneID 22913).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
The agouti (a) locus includes the mouse agouti gene that regulates coat color in mice by controlling the relative amount and distribution of yellow and black pigment in the coat hairs. A mutation in the agouti locus, lethal yellow (Ay), results in an all-yellow coat color along with a number of dominant pleiotropic effects such as obesity, diabetes, tumor susceptibility, and embryonic lethality in homozygotes. Molecular analysis of the Ay transcript revealed that the Ay transcript includes the coding region of the a gene but also included novel sequence. The novel sequence was found to originate from a 5’-noncoding exon of a gene that was named Raly, because it had the potential to encode an hnRNP protein that is associated with lethal yellow.
Alternate Names
autoantigen p542; heterogeneous nuclear ribonucleoprotein C-like 2; hnRNP associated with lethal yellow protein homolog; hnRNP core protein C-like 2; HNRPCL2; P542; RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog); RNA-binding protein (autoantigenic); RNA-binding protein (autoantigenic, hnRNP-associated with lethal yellow); RNA-binding protein RalY
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.