Rabbit anti-RbBP4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-206A maps to a region between residue 1 and 50 of human retinoblastoma binding protein 4 (retinoblastoma-binding protein p48) using the numbering given in entry CAA52321.1 (GeneID 5928).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RbBP4 immobilized on solid support.
The epitope recognized by A301-206A-T maps to a region between residue 1 and 50 of human retinoblastoma binding protein 4 (retinoblastoma-binding protein p48) using the numbering given in entry CAA52321.1 (GeneID 5928).
Additional Product Information
RbBP4 is a member of the WD-repeat subfamily of proteins and is a component of multiple complexes involved in chromatin assembly, histone deacetylation, and transcriptional repression. RbBP4 has also been identified as a retinoblastoma (Rb) binding protein and plays a role in the repression of E2F-regulated genes in association with Rb and histone deacetylases.
Alternate Names
CAF-1 subunit C; CAF-I 48 kDa subunit; CAF-I p48; chromatin assembly factor 1 subunit C; chromatin assembly factor I p48 subunit; chromatin assembly factor/CAF-1 p48 subunit; histone-binding protein RBBP4; lin-53; MSI1 protein homolog; nucleosome-remodeling factor subunit RBAP48; NURF55; RBAP48; RBBP-4; retinoblastoma-binding protein 4; retinoblastoma-binding protein p48
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.