Rabbit anti-RBM22 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
RNA binding motif protein 22 (RBM22) is involved in the first step of pre-mRNA splicing. It binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. RBM22 is involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NW64].
Alternate Names
Cwc2; fSAP47; functional spliceosome-associated protein 47; pre-mRNA-splicing factor RBM22; RNA-binding motif protein 22; ZC3H16; zinc finger CCCH domain-containing protein 16
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.