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Rabbit anti-RBM9 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
RBM9
Reactivity:
Human, Mouse, Rat
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications

Verified Reactivity
Rat, Mouse, Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to FOX2/RBM9 immobilized on solid support.

The epitope recognized by A300-864A maps to a region between residue 1 and 50 of human RNA Binding Motif Protein 9 using the numbering given in entry NP_055124.1 (GeneID 23543).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to RBM9 immobilized on solid support.

The epitope recognized by A300-864A-T maps to a region between residue 1 and 50 of human RNA Binding Motif Protein 9 using the numbering given in entry NP_055124.1 (GeneID 23543).
Country of Origin
USA

Additional Product Information

FOX2/RBM9 is an RNA binding protein thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. It binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts, and also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity.[RefSeq]

23543
RBFOX2
RNA binding fox-1 homolog 2
O43251
RNA binding protein fox-1 homolog 2

Alternate Names

dJ106I20.3; fox-1 homolog B; fox-1 homologue; FOX2; Fox-2; fxh; hexaribonucleotide-binding protein 2; HNRBP2; HRNBP2; RBM9; repressor of tamoxifen transcriptional activity; RNA binding protein fox-1 homolog 2; RNA binding protein, fox-1 homolog 2; RNA-binding motif protein 9; RNA-binding protein 9; RTA

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.