Rabbit anti-RCC2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-872A maps to a region between residue 1 and 50 of human Regulator of Chromosome Condensation 2 using the numbering given in entry NP_061185.1 (GeneID 55920).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RCC2 immobilized on solid support.
The epitope recognized by A300-872A-T maps to a region between residue 1 and 50 of human Regulator of Chromosome Condensation 2 using the numbering given in entry NP_061185.1 (GeneID 55920).
Additional Product Information
RCC2 (regulator of chromosome condensation 2) is a member of the RCC1 family of guanine nucleotide exchange factors. RCC2 has been identified as part of the chromosomal passenger complex (CPC) which coordinates the chromosomal and cytoskeletal events of mitosis. Chromosomal passengers are proteins present in complexes with aurora B, inner centromeric protein (INCENP), survivin, and borealin. RCC2 has been demonstrated to be required for the recruitment of survivin and aurora B and is essential for proper chromosome segregation and the completion of cell cleavage. Loss of RCC2 inhibits spindle assembly, activates the spindle assembly checkpoint, and blocks cells in prometaphase.
Alternate Names
epididymis secretory sperm binding protein; protein RCC2; RCC1-like protein TD-60; TD-60; telophase disk protein of 60 kDa
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.