Rabbit anti-RFWD3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-396A maps to a region between residue 75 and 125 of human ring finger and WD repeat domain 3 using the numbering given in entry NP_060594.3 (GeneID 55159).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RFWD3 immobilized on solid support.
The epitope recognized by A301-396A-T maps to a region between residue 75 and 125 of human ring finger and WD repeat domain 3 using the numbering given in entry NP_060594.3 (GeneID 55159).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
RFWD3 (ring finger and WD repeat domain 3) has been identified as a protein that is phosphorylated in response to DNA damage. The exact function of RFWD3 (ring finger and WD repeat domain 3) has not been characterized. Structurally, RFWD3 possesses a RING-type zinc finger and 3 WD repeats.
Alternate Names
E3 ubiquitin-protein ligase RFWD3; FANCW; RING finger and WD repeat domain-containing protein 3; RING finger protein 201; RING-type E3 ubiquitin transferase RFWD3; RNF201
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.