Rabbit anti-Ric-8A Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-572A maps to a region between residue 418 to 468 of human Resistance to Inhibitors of Cholinesterase 8 Homolog A using the numbering given in entry EAW61245.1 (GeneID 60626).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Ric-8A immobilized on solid support.
The epitope recognized by A304-572A-T maps to a region between residue 418 to 468 of human Resistance to Inhibitors of Cholinesterase 8 Homolog A using the numbering given in entry EAW61245.1 (GeneID 60626).
Additional Product Information
RIC8 guanine nucleotide exchange factor A (Ric-8A), also known as Synmebryn-A, is a guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. It is able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Ric-8A is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). It also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NPQ8].
Alternate Names
Protein Ric-8A; resistance to inhibitors of cholinesterase 8 homolog A; RIC8; synembryn-A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.