Rabbit anti-RNF213 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-841A maps to a region between residue 325 and 375 of human Protein ALO17 using the numbering given in entry Q63HN8.3 (GeneID 57674).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RNF213 immobilized on solid support.
The epitope recognized by A305-841A-T maps to a region between residue 325 and 375 of human Protein ALO17 using the numbering given in entry Q63HN8.3 (GeneID 57674).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
RNF213 is an E3 ubiquitin-protein ligase involved in angiogenesis. Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression. Also has ATPase activity. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q63HN8].
Alternate Names
ALK lymphoma oligomerization partner on chromosome 17; ALO17; C17orf27; E3 ubiquitin-protein ligase RNF213; KIAA1618; MYMY2; mysterin; MYSTR; NET57; RING finger protein 213; RING-type E3 ubiquitin transferase RNF213
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.