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Rabbit anti-RPL27/Ribosomal Protein L27 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
RPL27/Ribosomal Protein L27
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Dog, Chicken, Pig, Bovine, Rat
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to RPL27/Ribosomal Protein L27 immobilized on solid support.

The epitope recognized by A305-033A maps to a region between residue 25 to 75 of human 60S ribosomal protein L27 using the numbering given in entry P61353.2 (GeneID 6155).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to RPL27/Ribosomal Protein L27 immobilized on solid support.

The epitope recognized by A305-033A-T maps to a region between residue 25 to 75 of human 60S ribosomal protein L27 using the numbering given in entry P61353.2 (GeneID 6155).
Country of Origin
USA

Additional Product Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL27 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L27E family of ribosomal proteins. It is located in the cytoplasm [taken from NCBI Entrez Gene (Gene ID: 6155)].

6155
RPL27
ribosomal protein L27
P61353
60S ribosomal protein L27

Alternate Names

60S ribosomal protein L27; DBA16; L27; large ribosomal subunit protein eL27

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).