Rabbit anti-RPL7 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
RPL7 is a component of the 60S ribosomal subunit. Ribosomes are composed of a small 40S subunit and a large 60S subunit that function to catalyze protein synthesis. RPL7 is a member of the L30P family of ribosomal proteins and can bind both DNA and RNA in vitro. RPL7 is a cytoplasmic protein implicated to play a regulatory role in translation. It has also been identified as an auto-antigen in patients with autoimmune diseases such as lupus erythematosus.
Alternate Names
60S ribosomal protein L7; humL7-1; L7; large ribosomal subunit protein uL30
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.