Rabbit anti-RPS3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-840A maps to a region between residue 1 and 50 of human Ribosomal Protein S3 using the numbering given in entry NP_000996.2 (GeneID 6188).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RPS3 immobilized on solid support.
The epitope recognized by A303-840A-T maps to a region between residue 1 and 50 of human Ribosomal Protein S3 using the numbering given in entry NP_000996.2 (GeneID 6188).
Additional Product Information
RPS3 (40S ribosomal protein S3) has been identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. It has also been identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P23396].
Alternate Names
40S ribosomal protein S3; IMR-90 ribosomal protein S3; S3; small ribosomal subunit protein uS3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.