Rabbit anti-RPSA Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-298A maps to a region between residue 1 to 50 of human 40S ribosomal protein SA using the numbering given in entry P08865.4 (GeneID 3921).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RPSA immobilized on solid support.
The epitope recognized by A305-298A-T maps to a region between residue 1 to 50 of human 40S ribosomal protein SA using the numbering given in entry P08865.4 (GeneID 3921).
Additional Product Information
RPSA is required for the assembly and/or stability of the 40S ribosomal subunit. RPSA is required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. RPSA also functions as a cell surface receptor for laminin and plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. RPSA may play a role in cell fate determination and tissue morphogenesis. It acts as a PPP1R16B-dependent substrate of PPP1CA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08865].
Alternate Names
37 kDa laminin receptor; 37 kDa laminin receptor precursor; 37/67 kDa laminin receptor; 37LRP; 40S ribosomal protein SA; 67 kDa laminin receptor; 67LR; colon carcinoma laminin-binding protein; ICAS; LAMBR; Laminin receptor 1; laminin receptor 1 (67kD, ribosomal protein SA); laminin-binding protein precursor p40; lamR; LAMR1; LBP; LBP/p40; LRP; LRP/LR; multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4; p40; SA; small ribosomal subunit protein uS2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.