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Primary Antibodies  

Rabbit anti-S Tag Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog # A190-135A
Citations (6)
Target:
S Tag
Applications:
ELISA, ICC, IP, WB
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.1 mg)

A190-135A
$268.00
Qty:
Bulk Request

Product Details

Specifications

Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Production Procedures
Rabbits were immunized with S-Tag (KETAAAKFERQHMDS) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

The S tag is derived from pancreatic ribonuclease A, and has a primary amino acid sequence of KETAAAKFERQHMDS. An S tag can be used for WB, IP, and microscopic purposes.

Applications

Not all listed applications have been specifically tested by our laboratory. All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.