Rabbit anti-SART1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-422A-T maps to a region between residue 675 and 725 of human squamous cell carcinoma antigen recognized by T cells using the numbering given in entry NP_005137.1 (GeneID 9092).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SART1 immobilized on solid support.
The epitope recognized by A301-422A maps to a region between residue 675 and 725 of human squamous cell carcinoma antigen recognized by T cells using the numbering given in entry NP_005137.1 (GeneID 9092).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
SART1 (squamous cell carcinoma antigen recognized by T cells 1) was originally identified as a gene that encodes an antigenic peptide expressed in squamous cell carcinomas and recognized by cytotoxic T lymphocytes. The SART1 gene encodes two proteins, SART1(800) and SART1(259). SART1(800) has been found to be expressed in the nucleus of the majority of proliferating cells and has been identified as a component of the splicesomal complex required for U4/U6.U5 tri-snRNP recruitment and the assembly of mature splicesomes.
Alternate Names
allergen Hom s 1; Ara1; HAF; HOMS1; hSART-1; hSnu66; hypoxia associated factor; IgE autoantigen; SART-1; SART1(259) protein; SART1(800) protein; SART1, U4/U6.U5 tri-snRNP-associated protein 1; SART1259; small nuclear ribonucleoprotein 110kDa (U4/U6.U5); SNRNP110; Snu66; SNU66 homolog; squamous cell carcinoma antigen recognised by T cells; squamous cell carcinoma antigen recognized by T-cells 1; U4/U6.U5 tri-snRNP-associated 110 kDa protein; U4/U6.U5 tri-snRNP-associated protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.