Rabbit anti-SCC-112 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-089A maps to a region between residue 1250 and the C-terminus (residue 1297) of human SCC-112 using the number given in entry NP_056015.1 (GeneID 23244).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SCC-112 immobilized on solid support.
The epitope recognized by A300-089A-T maps to a region between residue 1250 and the C-terminus (residue 1297) of human SCC-112 using the number given in entry NP_056015.1 (GeneID 23244).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
SCC-112, also known as Pds5A, interacts with the cohesin multisubunit complex that plays a critical role in chromosome organization, segregation, gene regulation, and DNA repair. Pds5 is implicated to participate in both stabilization and destabilization of cohesion-mediated cohesion in mitotic chromosomes.
Alternate Names
cell proliferation-inducing gene 54 protein; PDS5, regulator of cohesion maintenance, homolog A; PIG54; SCC112; SCC-112; sister chromatid cohesion protein 112; sister chromatid cohesion protein PDS5 homolog A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.