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Rabbit anti-SEC22B Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
SEC22B
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 100 and 150
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse, Chicken, X. tropicalis, Zebrafish, Rat
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to SEC22B immobilized on solid support.

The epitope recognized by A304-601A maps to a region between residue 100 to 150 of human SEC22 Vesicle Trafficking Protein Homolog B using the numbering given in entry NP_004883.3 (GeneID 9554).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

SEC22B is a member of the SEC22 family of vesicle trafficking proteins. It seems to complex with SNARE and it is thought to play a role in the ER-Golgi protein trafficking. SEC22B has strong similarity to Mus musculus and Cricetulus griseus proteins [taken from NCBI Entrez Gene (Gene ID: 9554)].

9554
SEC22B
SEC22 homolog B, vesicle trafficking protein (gene/pseudogene)
O75396
Vesicle-trafficking protein SEC22b

Alternate Names

ER-Golgi SNARE of 24 kDa; ERS24; ERS-24; SEC22 vesicle trafficking protein homolog B; SEC22 vesicle trafficking protein-like 1; SEC22 vesicle-trafficking protein homolog B; SEC22 vesicle-trafficking protein-like 1; SEC22L1; vesicle-trafficking protein SEC22b

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.