Bethyl Laboratories

Rabbit anti-SEC24C Antibody Affinity Purified

Primary
Catalog #A304-760A
TargetSEC24C
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Detection of human SEC24C by western blot of immunoprecipitates.
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Product Description

SEC24-related protein C (SEC24C) is a component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P53992].

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 275 and 325
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSEC24C
Antigen Species
Human
Gene ID9632
Gene Symbol
SEC24C
Gene Name
SEC24 homolog C, COPII coat complex component
Uniprot IDP53992
Protein Name
Protein transport protein Sec24C
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to SEC24C immobilized on solid support.

The epitope recognized by A304-760A maps to a region between residue 275 to 325 of human Protein Transport Protein Sec24C using the numbering given in entry P53992.3 (GeneID 9632).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunoprecipitation (IP)6 µg/mg lysate

Documents

Product DatasheetPDF · A304-760A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-760A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-760AOpen

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