Rabbit anti-SENP1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-927A maps to a region between residue 1 and 50 of human SUMO1/Sentrin Specific Peptidase 1 using the numbering given in entry NP_055369.1 (GeneID 29843).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SENP1 immobilized on solid support.
The epitope recognized by A302-927A-T maps to a region between residue 1 and 50 of human SUMO1/Sentrin Specific Peptidase 1 using the numbering given in entry NP_055369.1 (GeneID 29843).
Additional Product Information
SENP1 (sentrin-specific protease) functions as a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugation of SUMO1 from HDAC1 decreases its transcriptional repression activity. SENP1 also deconjugates SUMO1 from HIPK2 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9P0U3].
Alternate Names
sentrin/SUMO-specific protease SENP1; sentrin-specific protease 1; SUMO1/sentrin specific peptidase 1; SUMO1/sentrin specific protease 1; SuPr-2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.