Rabbit anti-SENP3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-140A maps to a region between residue 275 and 325 of human SUMO1/Sentrin/SMT3 Specific Peptidase 3 using the numbering given in entry NP_056485.2 (GeneID 26168).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SENP3 immobilized on solid support.
The epitope recognized by A303-140A-T maps to a region between residue 275 and 325 of human SUMO1/Sentrin/SMT3 Specific Peptidase 3 using the numbering given in entry NP_056485.2 (GeneID 26168).
Additional Product Information
Sentrin-specific protease 3 (SENP3) functions as a protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates.SENP3 deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability, and also deconjugates SUMO2 and SUMO3 from CDCA8. SENP3 can function as a redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. SENP3 is required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9H4L4].
Alternate Names
sentrin/SUMO-specific protease 3; Sentrin/SUMO-specific protease SENP3; sentrin-specific protease 3; SMT3IP1; SSP3; SUMO1/sentrin/SMT3 specific peptidase 3; SUMO-1-specific protease 3; Ulp1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.