Rabbit anti-SF3A2/SAP62 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-821A maps to a region between residue 1 to 50 of human Splicing factor 3A subunit 2 using the numbering given in entry Q15428.2 (GeneID 8175).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SF3A2/SAP62 immobilized on solid support.
The epitope recognized by A304-821A-T maps to a region between residue 1 to 50 of human Splicing factor 3A subunit 2 using the numbering given in entry Q15428.2 (GeneID 8175).
Additional Product Information
SF3A2 is subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein [taken from NCBI Entrez Gene (Gene ID: 8175)].
Alternate Names
pre-mRNA splicing factor SF3A, subunit 2; PRP11; PRPF11; SAP 62; SAP62; SF3a66; spliceosome associated protein 62; Spliceosome-associated protein 62; splicing factor 3A subunit 2; splicing factor 3a, subunit 2, 66kD; splicing factor 3a, subunit 2, 66kDa
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.