Rabbit anti-SF3A3 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
SF3A3 is one of 3 subunits of the SF3a splicing factor. SF3a is composed of a 60 kDa subunit (SF3A3/SF3a60), 66 kDa subunit (SF3A2/SF3a66), and a120 kDa subunit (SF3A1/SF3a120). SF3a is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing [taken from NCBI Entrez Gene (GeneID: 10946)].
Alternate Names
pre-mRNA splicing factor SF3a (60kD); PRP9; PRPF9; SAP 61; SAP61; SF3a60; spliceosome associated protein 61; Spliceosome-associated protein 61; splicing factor 3A subunit 3; splicing factor 3a, subunit 3, 60kD; splicing factor 3a, subunit 3, 60kDa
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.