Rabbit anti-SF3b155/SAP155 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-997A maps to a region between residue 200 and 250 of human splicing factor 3b, subunit 1, 155kDa using the numbering given in entry NP_036565.2 (GeneID 23451).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SF3b155/SAP155 immobilized on solid support.
The epitope recognized by A300-997A-T maps to a region between residue 200 and 250 of human splicing factor 3b, subunit 1, 155kDa using the numbering given in entry NP_036565.2 (GeneID 23451).
Additional Product Information
SF3B155 is a subunit of the U2 small nuclear ribonucleoproteins complex (U2 snRNP) that functions in pre-mRNA splicing. SF3B155 together with splicing factor 3a binds pre-mRNA upstream of the branchpoint sequence to anchor U2 snRNP to the pre-mRNA. SF3B155 is also a component of the U12-type splicesome that is involved in the splicing of a rare class of nuclear pre-mRNA introns.
Alternate Names
Hsh155; MDS; pre-mRNA processing 10; pre-mRNA splicing factor SF3b, 155 kDa subunit; Pre-mRNA-splicing factor SF3b 155 kDa subunit; PRP10; PRPF10; SAP 155; SAP155; SF3b155; spliceosome-associated protein 155; splicing factor 3B subunit 1; splicing factor 3b, subunit 1, 155kDa
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.