Rabbit anti-SF3B4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-950A maps to a region between residue 374 to 424 of human Splicing Factor 3b, Subunit 4, 49kDa using the numbering given in entry NP_005841.1 (GeneID 10262).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SF3B4 immobilized on solid support.
The epitope recognized by A303-950A-T maps to a region between residue 374 to 424 of human Splicing Factor 3b, Subunit 4, 49kDa using the numbering given in entry NP_005841.1 (GeneID 10262).
Additional Product Information
Splicing factor 3b, subunit 4, 49kDa (SF3B4) is one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B [taken from NCBI Entrez Gene (Gene ID: 10262)].
Alternate Names
AFD1; Hsh49; pre-mRNA-splicing factor SF3b 49 kDa subunit; SAP 49; SAP49; SF3b49; SF3b50; spliceosomal protein; spliceosome-associated protein (U2 snRNP); spliceosome-associated protein 49; splicing factor 3B subunit 4; splicing factor 3b, subunit 4, 49kD; splicing factor 3b, subunit 4, 49kDa
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.