Rabbit anti-SFRS14 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-607A maps to a region between residue 725 and 775 of human splicing factor, arginine/serine-rich 14 using the numbering given in entry NP_055699.1 (GeneID 10147).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SFRS14 immobilized on solid support.
The epitope recognized by A301-607A-T maps to a region between residue 725 and 775 of human splicing factor, arginine/serine-rich 14 using the numbering given in entry NP_055699.1 (GeneID 10147).
Additional Product Information
SFRS14 (splicing factor arginine/serine-rich 14) is a putative alternative splicing factor. It contains two SURP motif repeats which are found among proteins involved in regulated mRNA splicing and a G-patch domain which features six highly conserved glycine residues found in several RNA-associated proteins. SFRS14 also bears an N-terminal arginine/serine-rich region found among the SR-related family of pre-mRNA processing factors.
Alternate Names
arginine/serine-rich-splicing factor 14; SFRS14; splicing factor, arginine/serine-rich 14; SRFS14; SURP and G-patch domain-containing protein 2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.