Rabbit anti-SIL Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
SCL-interrupting locus (SIL) plays an important role in embryonic development as well as in cellular growth and proliferation. The long-term silencing of SIL affects cell survival and cell cycle distribution as well as decreases CDC2 activity correlated with reduced phosphorylation of CDC2. SIL is a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15468].
Alternate Names
MCPH7; SCL/TAL1 interrupting locus; SCL-interrupting locus protein; SIL; TAL-1-interrupting locus protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.