Rabbit anti-SIPA1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-028A maps to a region between residue 992 to 1042 of human signal-induced proliferation-associated protein 1 using the numbering given in entry NP_006738.3 (GeneID 6494).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SIPA1 immobilized on solid support.
The epitope recognized by A302-028A-T maps to a region between residue 992 to 1042 of human signal-induced proliferation-associated protein 1 using the numbering given in entry NP_006738.3 (GeneID 6494).
Additional Product Information
The regulation of GTPase activity is achieved via activators called GEFs (guanine-nucleotide exchange factors) and inactivators called GAPs (GTPase-activating proteins). Signal-induced proliferation-associated protein 1 (SIPA1) is a GTPase activating protein (GAP) for the Rap1 and Rap2 small GTPases. Spa-1 has been found to play an important role in hematopoietic cell development and the invasion and metastasis of human cancers.
Alternate Names
GTPase-activating protein Spa-1; p130 SPA-1; signal-induced proliferation-associated gene 1; signal-induced proliferation-associated protein 1; sipa-1; SPA1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.