Rabbit anti-SKP2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-437A-T maps to a region between residue 50 and 100 of human S-phase kinase-associated protein 2 using the numbering given in entry NP_005974.2 (GeneID 6502).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SKP2 immobilized on solid support.
The epitope recognized by A302-437A maps to a region between residue 50 and 100 of human S-phase kinase-associated protein 2 using the numbering given in entry NP_005974.2 (GeneID 6502).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
S-phase kinase-associated protein 2 (SKP2) is a member of the F-box family of proteins. It is the substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. SKP2 specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13309].
Alternate Names
CDK2/cyclin A-associated protein p45; Cyclin-A/CDK2-associated protein p45; FBL1; F-box protein Skp2; F-box/LRR-repeat protein 1; FBXL1; FLB1; p45; p45skp2; S-phase kinase-associated protein 2; S-phase kinase-associated protein 2, E3 ubiquitin protein ligase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.