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Rabbit anti-SLK Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
SLK
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Guinea Pig, Mouse, Rat
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to SLK immobilized on solid support.

The epitope recognized by A300-499A maps to a region between residues 1 and 50 of human Ste20-like Kinase using the numbering given in entry NP_055535.2 (GeneID 9748).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Ste20-like kinase (SLK) plays a role in cytoskeletal organization during cell spreading and can induce actin stress fiber disassembly. SLK is a serine/threonine kinase that is thought to be regulated by casein kinase 2 (CK2) and is also involved in apoptosis.

9748
SLK
STE20 like kinase
Q9H2G2
STE20-like serine/threonine-protein kinase

Alternate Names

bA16H23.1; CTCL tumor antigen se20-9; hSLK; Long Ste20-like Kinase; LOSK; se20-9; serine/threonine-protein kinase 2; SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase, SNF1 sucrose nonfermenting like kinase; SNF1 sucrose nonfermenting like kinase; STE20-like kinase; STE20-like serine/threonine-protein kinase; STE20-related kinase; Ste20-related serine/threonine kinase; STE20-related serine/threonine-protein kinase; STK2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.