Rabbit anti-SMARCA3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-230A maps to a region between residues 600 and 700 of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3 using the number given in entry NP_003062.2 (GeneID 6596).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SMARCA3 immobilized on solid support.
The epitope recognized by A300-230A-T maps to a region between residues 600 and 700 of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3 using the number given in entry NP_003062.2 (GeneID 6596).
Additional Product Information
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SMARCA3 also known as HLTF (helicase-like transcription factor) has been shown to bind DNA promoter elements and stimulate transcription in cooperation with Sp1and Sp3 transcription factors.
Alternate Names
DNA-binding protein/plasminogen activator inhibitor 1 regulator; DNA-binding protein/plasminogen activator inhibitor-1 regulator; helicase-like transcription factor; HIP116; HIP116A; HLTF1; RING finger protein 80; RING-type E3 ubiquitin transferase HLTF; RNF80; SMARCA3; SNF2L3; SNF2-like 3; sucrose nonfermenting protein 2-like 3; sucrose nonfermenting-like 3; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; ZBU1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.