Rabbit anti-SMARCD2/BAF60b Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-596A maps to a region between residue 425 and 475 of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2 (mammalian chromatin remodeling complex BRG1-associated factor 60B) using the numbering given in entry NP_003068.2 (GeneID 6603).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SMARCD2/BAF60b immobilized on solid support.
The epitope recognized by A301-596A-T maps to a region between residue 425 and 475 of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2 (mammalian chromatin remodeling complex BRG1-associated factor 60B) using the numbering given in entry NP_003068.2 (GeneID 6603).
Additional Product Information
The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. SMARCD2/BAF60B was isolated from a cDNA library in a search for proteins similar to mouse BAF60A which was identified as the paralog of the S. cerevisiae SWI/SNF component, SWP73.
Alternate Names
60 kDa BRG-1/Brm-associated factor subunit B; BAF60B; BRG1-associated factor 60B; chromatin remodeling complex BAF60B subunit; CRACD2; mammalian chromatin remodeling complex BRG1-associated factor 60B; PRO2451; Rsc6p; SGD2; SWI/SNF complex 60 kDa subunit B; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2; Swp73-like protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).