Rabbit anti-SMG5 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-804A-T maps to a region between residue 400 and 450 of human Suppressor with Morphogenetic Effects on Genitalia 5 using the numbering given in entry NP_056142.2 (GeneID 23381).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SMG5 immobilized on solid support.
The epitope recognized by A302-804A maps to a region between residue 400 and 450 of human Suppressor with Morphogenetic Effects on Genitalia 5 using the numbering given in entry NP_056142.2 (GeneID 23381).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
SMG5 (Suppressor with Morphogenetic Effects on Genitalia 5) Plays a role in nonsense-mediated mRNA decay. SMG5 promotes dephosphorylation of RENT1 and is necessary for TERT activity [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9UPR3].
Alternate Names
EST1 telomerase component homolog B; EST1B; EST1-like protein B; Est1p-like protein B; ever shorter telomeres 1B; hSMG-5; LPTS interacting protein; LPTS-interacting protein; LPTSRP1; LPTS-RP1; protein SMG5; SMG-5; SMG-5 homolog; smg-5 homolog, nonsense mediated mRNA decay factor
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.