Rabbit anti-SMG9 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-210A-T maps to a region between residue 75 and 125 of human suppressor with morphogenetic effect on genitalia protein 9 using the numbering given in entry NP_061981.2 (GeneID 56006).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SMG9 immobilized on solid support.
The epitope recognized by A302-210A maps to a region between residue 75 and 125 of human suppressor with morphogenetic effect on genitalia protein 9 using the numbering given in entry NP_061981.2 (GeneID 56006).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
SMG9 is a subunit of the SMG-1 complex. SMG-1 and Upf1 are part of a surveillance complex that participates in nonsense-mediated mRNA decay (NMD) to degrade mRNAs containing premature translation initiation codons (PTCs).
Alternate Names
C19orf61; F17127_1; HBMS; protein SMG9; Protein SMG-9; protein smg-9 homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.