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Rabbit anti-SMG9 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
SMG9
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 75 and 125
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse, Bovine, Rat
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to SMG9 immobilized on solid support.

The epitope recognized by A302-210A-T maps to a region between residue 75 and 125 of human suppressor with morphogenetic effect on genitalia protein 9 using the numbering given in entry NP_061981.2 (GeneID 56006).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to SMG9 immobilized on solid support.

The epitope recognized by A302-210A maps to a region between residue 75 and 125 of human suppressor with morphogenetic effect on genitalia protein 9 using the numbering given in entry NP_061981.2 (GeneID 56006).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

SMG9 is a subunit of the SMG-1 complex. SMG-1 and Upf1 are part of a surveillance complex that participates in nonsense-mediated mRNA decay (NMD) to degrade mRNAs containing premature translation initiation codons (PTCs).

56006
SMG9
SMG9, nonsense mediated mRNA decay factor
Q9H0W8
Protein SMG9

Alternate Names

C19orf61; F17127_1; HBMS; protein SMG9; Protein SMG-9; protein smg-9 homolog

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.